Phylogenic sampling across Onagraceae will investigate diversification and test evolutionary hypotheses associated with trait variation. Similar approaches will assess host specificity, uncover new species and patterns of diversification of Mompha associated with Onagreae. Like other species-rich plant genera (e.g. Penstemon, Castilleja), Oenothera has diversified across the mountain, grassland and desert habitats of North America with conservative pollinator shifts (mostly between bees and moths), but more dramatic shifts in growth form and life history traits. These shifts should have had profound effects on the ecology and evolution of Mompha moths, determining voltinism, host specificity and feeding niche. Our broad sampling is designed to capture as much of the extant remnants of their codiversification as is feasible.
We propose here to use the genomic resources of this project to develop two sets of ~500 nuclear markers that will be used to improve resolution for the phylogeny of Onagreae and Mompha. Transcriptomes for six species of Mompha and three species of Oenothera will be used to develop two set of baits for liquid-phase DNA capture (Hyb-Seq, or sequence enrichment). Hyb-Seq is a cost-effective way of sequencing a high volume of markers for many taxa, and can provide several million sites for phylogenetic reconstruction.